Minyak Atsiri Indonesia

Abstrak Penelitian

ABSTRAK PENELITIAN BUDIDAYA BANGLE

PENGARUH PEMBERIAN PACLOBUTRAZOL TERHADAP PERTUMBUHAN BANGLE (Zingiber purpureum Roxb) DALAM PENYIMPANAN IN –VITRO

Oleh: Meynarti Sari Dewi Ibrahim; Balai Penelitian Tanaman Rempah dan Obat

Buletin TRO Vol. XVI, No. 2, 2005

ABSTRAK

Penelitian pengaruh pemberian paclobutrazol terhadap pertumbuhan bangle dalam penyimpanan secara in vitro, dilakukan di laboratorium Plasma Nutfah dan Pemuliaan Balai Penelitian Tanaman Rempah dan Obat dari bulan Februari sampai November 2003. Eksplan yang digunakan adalah anakan tunas yang telah steril. Perlakuan yang diuji adalah paclobutrazol dengan konsentrasi 0 (kontrol); 1; 2 dan 3 mg/l. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) dengan 10 ulangan. Penelitian ini dilakukan untuk mengetahui pengaruh pemakaian zat penghambat tumbuh paclobutrazol terhadap pertumbuhan tunas bangle dalam penyimpanan secara in vitro. Hasil penelitian menunjukkan bahwa pemberian paclobutrazol berpengaruh nyata terhadap jumlah tunas, tinggi tanaman, jumlah daun dan jumlah akar. Pada umur 5 bulan semakin tinggi konsentrasi paclobutrazol jumlah tunas, daun, dan akar semakin rendah tanaman menjadi pendek. Pemberian Paclobutrazol 2 mg/l dan 3 mg/l dapat memperpanjang masa simpan sampai 9 bulan. Penampilan visual planlet memperlihatkan pemberian paclobutrazol dapat mempertegar batang sehingga planlet terlihat lebih kokoh.

Kata kunci : Bangle (Zingiber purpureum Roxburgh), paclobutrazol, penyimpanan In Vitro

ABSTRACT

Effect of Paclobutrazol On The Growth of In Vitro Concerved Purple Ginger (Zingiber purpureum Roxb)
Effect of paclobutrazol on the growth of Purple Ginger (Zingiber purpureum Roxburgh) conserved in vitro were studied in the Laboratory of Breeding and Gremplasm of Indonesian Spice and Medicinal Crops Research Instituted, from February to November 2003. Sterile shoots of Purple Ginger were used as explants sources. The treatments evaluated were paclobutrazol 0 (control); 1; 2; 3 mg/l and arranged in completely randomized design with 10 replications. The aim of research was to study the effect of paclobutrazol on the growth of Purple Ginger conserved in vitro. Results showed that at 5 months of culture age, paclobutrazol significantly reduced the number of shoots, height number of leaves and number of roots. The higher the concentration of paclobutrazol, the lower the number of shoots, leaves and roots and the shorter the root of plantlets observed. Application of paclobutrazol at concentration of 2 or 3 mg/l could prolong the conservation period up to 9 months. Fur there more it was revealed that paclobutrazol application produced more vigorous plantlets.

Key words : Purple Ginger (Zingiber purpureum Roxburgh), paclobutrazol, in vitro

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